肠炎沙门菌luxS缺失株的构建及其生物学特性研究

doi:10.11843/j.issn.0366-6964.2018.06.022

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (6): 1291-1298.doi: 10.11843/j.issn.0366-6964.2018.06.022

肠炎沙门菌luxS缺失株的构建及其生物学特性研究

于艳超¹, 王莉莉², 潘巧², 辛九庆², 王秀梅^2*

1. 吉林农业大学动物科学技术学院, 长春 130118;
2. 中国农业科学院哈尔滨兽医研究所动物支原体创新团队, 哈尔滨 150069

收稿日期:2017-11-14 出版日期:2018-06-23 发布日期:2018-06-23
通讯作者: 王秀梅,助研,E-mail:xmwang99@163.com
作者简介:于艳超(1992-),男,吉林农安人,硕士,主要从事细菌耐药机制的研究,E-mail:yuyanchao0812@163.com
基金资助:
国家自然科学基金青年科学基金项目（31402249）

Construction and Biological Characteristics of Salmonella Enterica Serovar Enteritidis luxS Mutant

YU Yan-chao¹, WANG Li-li², PAN Qiao², XIN Jiu-qing², WANG Xiu-mei^2*

1. Animal Science and Technology College, Jilin Agricultural University, Changchun 130118, China;
2. Animal Mycoplasma Innovation Team, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China

Received:2017-11-14 Online:2018-06-23 Published:2018-06-23

摘要/Abstract

摘要：

拟研究LuxS/AI-2群体感应系统对肠炎沙门菌（Salmonella enterica Serovar Enteritidis，SE）生物学特性的影响。利用Red同源重组系统构建LuxS/AI-2系统关键基因luxS缺失株，通过比较亲本株和luxS缺失株体外生长速率、药物敏感性、细胞黏附侵袭能力及生物被膜形成能力等生物学特性，初步分析AI-2/LuxS系统对SE生物学特性的影响。结果显示，luxS缺失株能够稳定遗传缺失的△luxS基因；体外生长速率略快；生物被膜形成能力明显增强；对DF-1细胞和Caco-2细胞黏附侵袭能力均显著增加；对氟喹诺酮类药物敏感性提高128~256倍，对氨苄西林、四环素、多西环素、氯霉素和氟苯尼考类等的药物敏感性提高了8倍。以上结果表明，LuxS系统影响肠炎沙门菌的生物学特性（如生物被膜形成、对细胞的黏附侵袭能力），并且该系统也通过某种耐药机制影响肠炎沙门菌的药物敏感性。

Abstract:

The study was conducted to investigate the effect of LuxS/AI-2 quorum-sensing system on the biological characteristics of Salmonella enterica Serovar Enteritidis. A luxS mutant strain was constructed by Red homologous recombination system, and its ΔluxS gene stability, growth features, antimicrobial susceptibility, adherence and invasion, as well as biofilm formation were determined and compared with the wild type. Our results showed that the ΔluxS gene exhibits stability in ΔluxS mutant. The growth of ΔluxS mutant was slightly faster than the wild strain in vitro. Biofilm formation of the mutant strain was significantly enhanced as well as the adhesion and invasion ability to DF-1 cells and Caco-2 cells. Deletion of luxS resulted in a 128-to 256-fold increase in susceptibility to fluoroquinolones and moderate increases (2-to 8-fold) in susceptibility to ampicillin, tetracycline, doxycycline, chloramphenicol, and florfenicol. These results suggest that LuxS/AI-2 system affects biological characteristics of SE including biofilm formation, adhesion and invasion to cells, moreover, the AI-2/LuxS sensing system affect antimicrobial susceptibility of SE through certain resistance mechanisms.

中图分类号:

S852.612

于艳超, 王莉莉, 潘巧, 辛九庆, 王秀梅. 肠炎沙门菌luxS缺失株的构建及其生物学特性研究[J]. 畜牧兽医学报, 2018, 49(6): 1291-1298.

YU Yan-chao, WANG Li-li, PAN Qiao, XIN Jiu-qing, WANG Xiu-mei. Construction and Biological Characteristics of Salmonella Enterica Serovar Enteritidis luxS Mutant[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1291-1298.

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肠炎沙门菌luxS缺失株的构建及其生物学特性研究

Construction and Biological Characteristics of Salmonella Enterica Serovar Enteritidis luxS Mutant

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